Insemenation rank

Ekco

So my af was sept 7th and I got a positive opk on sept 25. I was only able to do one insemenation on the 24th late that night. So that means I probably didn’t ovulation till 2 days after my pos opk which would been the 27th or 28th right? I’m so annoyed 😒 so I can assume I missed it right?