Fmu first morning urine

Kellie • Mother of three and currently ttc #4 due to health issues my chances are starting to shorten every day! Staying positive and wishing everyone lots of baby dust
So I've got one more hpt to use and I said I want buying anymore lol using first morning urine does that mean I need to hold it for a little while or just go soon as I wake up! I've heard women say they held their pee for an hour then peed on the stick..