Confused

So I took these Monday,they clearly have a line. They sat for like 5 minutes or so but I took one today(pink dye $1 test) and it was negative. Now here’s the part I have a question on. I peed in a cup and left in on my bathroom counter this morning(yes it was my first morning pee) and had to run some errands so I didn’t get to put the pee in the test for at least 3 hours. Would that mess with results? I didn’t refrigerate the urine before I left.