One shot at inseminiting, when do I do it?

I have one vial of donor sperm, and am unsure when I should inseminate based on ovulation predictor tests. I’m planning to use preseed and a soft cup as well (using the syringe to deposit the sample, then placing the cup) if you’ve got any input please share! Really wanting this cycle to work but all of the things I’m trying are new to me.

Vote below to see results!