When is the earliest you can know you aren’t pregnant?

Okay this might be a weird question and maybe I’m just uneducated in this department.. but I’m about 3dpo, is it possible to know you aren’t pregnant at that point? I get a whiff of this sort of strong maybe iron smell coming from down there as early as yesterday (2dpo). What happens to our bodies at this point in our cycle? Could this be an extremely early sign that I’m not pregnant? Cause I definitely consider it that but maybe I’m just being paranoid. Also typically my nipples will start getting tender on 4dpo.