How much does time of day actually matter for OPKs? The instructions and Glow say that late morning/early afternoon are the best time to test. I've been testing first thing in the morning because that's when I'm most likely to be consistent when I test. (Just got off BCP last month after using it for eons so I'm testing every day. So far no sign of ovulation and AF is late but it's probably just my body being super confused - 2 negative pregnancy tests.) Another reason I've been testing in the morning is that the ideal time frame is when I'm at work. 😕