irregular periods - when should I test?

S
My periods are irregular (the longest 31 days, shortest 19 days), so I am not sure when to take a pregnancy test. I only had sex once this month on the 14th April (CD16), which would be approximately when I would ovulate. I am now on CD 33 and no AF and pregnancy test is negative. Would this be a definate negative or is there a possibility I am pregnant? I only ask because I have early pregnancy symptoms (Cervix: High-Soft-Closed, cramps, nausea, headaches, fatigue, back ache and fuller boobs - but they don't hurt, loss of appetite and achey legs). 
If there is a possibility that I could be pregnant when would you advise I take another test?