this is my first time <a href="https://play.google.com/store/apps/details?id=com.glow.android">tracking ovulation</a> and I’m unsure if this is normal the darkness of my lines vary and I hold my urine atleast 2 hrs prior to testing I work night shift so that’s why my times are at crazy hours not sure if night shift affects hormone levels so I’ve been testing at all hours just not with first urine after sleeping. My cycle is about 25 days with 6 says of AF cycle day 9 at 8 am has been my darkest (just came home from night shift so had been up all night) but I still think it was a shade lighter than control did I miss ovulation? Or is it normal for shades to vary?