FMU vs a 2 hr hold

Can a short hold affect the accuracy of a test?

Or should it still show regardless?

I had a VFL 12dpo and again on 13dpo but lighter. I drank a bottle of water at 11, peed at 11:30 then held my urine until 2 when I tested again. Could this be why it was fainter the 2nd time? Thanks!! So nervous.