When to do an ovulation test?

Kayley
My husband and I are TTC. This will be our 3rd month - I know we've not been trying that long but I'm 30 now and would like to get pregnant this year - and it's so disheartening getting a period, I want to try and 'get it right' ASAP.
Anyway, we got an ovulation test kit today, with 10 tests. Glow predicts I will be ovulating between the 16th-22nd Feb. When would be the best time to take my first test - and if that doesn't prove conclusive, when should I take the 2nd?
Also, does it matter if it's my first wee of that day? 
Thank you:)