Weird thing happened...

I took a hpt today, and there was a vvf line, I was going to take a pic, but the light in my bathroom was yellowish so I wanted to take the pic in a different room.  About that time my dog started barking so I just scooped it up quickly and ran to get my dog.  I noticed I picked it up by the urine part(uhmm yuck) and when I did it caused more urine to run across the test, then it appeared negative.  Planning on taking another one but trying not to get my hopes up. ETA:  could too much urine have caused the line to go away?