Only 1 day +OPK

Hi! Wondering if anyone has gotten pregnant in a similar situation...I usually O on day 13 or 14 (according to charting. This is the first month I used opk. I did one Sat night around 9pm=neg (had sex twice this day). Did one yesterday at 2pm= positive. Had sex last night. Took another test today around 2pm, just out of curiousity (I know u really don't have to take another one once u get a positive) and it was negative. My questions is was having sex last night enough??? Our schedules won't permit us to have sex for the next two days!!! 😏