I would recommend use them for a full cycle just to see how YOUR hormones ebb and flow. Start with first day after your period, testing once a day. Then depending on how long your cycles are starting testing twice a day about 6 days before mid-cycle. When you see the peak switch back to testing once a day until your period. For the once a day, I tested in mornings but some women get their LH rise in the evenings thus the testing twice a day closer to predicted ovulation.But really it is at your convenience. I did it when I woke up then again before bed.

I usually test around 10am then again in the evening sometime once my tests look close to positive. You can test pretty much anytime but they says with OPKs it’s best NOT to use the first morning pee. When the line starts getting darker and you’re getting close to ovulation, test at least twice a day so that you catch your peak!

I always tested between 2-4 pm and rhen again between 6-8 pm if my tests were getting close to positive.