Question on sperm

Annelise • Mom of 5
How long should you wait to have sex again for the sperm to mature ? 
This week is the week I should be ovulating. My husband an I had sex Saturday night and last night. Yesterday was the first day of my fertile week. I know sperm can live up to 48 hours so should we not have sex tonight ? I'm curious on if others just have sex every other night during this week? Or every night, every two nights etc. we are TTC #2 and with our first one we managed to get pregnant with only having sex twice during my fertile week.