When to test? Read Description!

I’ve read over and over that you should use FMU when testing. However for the past three nights I haven’t been able to stay asleep and I’ve been taking melatonin. So I have woken up around 1 am for the past three nights and stayed awake for a half an hour to two hours. During this time I usually have to pee and that’s what wakes me up. So I go pee and then I test when I wake up for work at 5 am. Would you say I need to test when I wake up the first time (1:00 am) or the second time (5:00 am)? 🧐