Vvfl now nothing ?

On Friday morning I got a VVVVFL but it was there enough to see without squinting . Now I’m getting negatives ( maybes a slight line but not as visible ) I’m nauseas , my breasts are so heavy and tender . But I’m not seeing a line on the tests . Does this mean there is no way I am pregnant ?

Out of the 19 months ttc this is the first time I had ever seen a second line so I was very excited at the idea that this could finally be it. Has anyone else had this happen but still been pregnant?