Ahhhh confused ! Still a chance ??

Lori

So I peed on the lighter line looking stick last night around 12am so I BD like two hours later around 2-3am and used preseed as well. I retested my ovulation this afternoon when I woke up at 1pm and the line was darker.

My question is how long do I have to BD again and will it still count since I had sex late last night??