Silly question

I've never taken a pregnancy test before but I do need to and I have always read other posts on here suggesting to others that your morning urine is the best to test with but I have never read much into it because I have never needed to until now. I don't know if this is a silly question or not, but if I work graves is it best to still try testing in the morning or at night during my morning? Hopefully that makes sense because I'm not sure I'm understanding all of it. I'm also confused on how to figure out the dpo.. I've read up on it and done the calculations myself but have also tried several different generated ovulation calculators online and I get different dates :/ my last period was August 20-24 and I figured September 3 as my ovulation date, soo my husband and I tried both September 1 and 4. When is the best time to test?