When should I start testing?

In 2018 we tried for my daughter and my first cycle off bc, it was a 43 day cycle without tracking OPKs. We conceived on the cycle with positive opk on cd18 with ovulation around cd19/20, and positive hpt 9/10 dpo, but would have been the start of AF based on my usual 28day cycle.

This time around, I’m on cd39, and got my first opk positive on 6/2 with a peak on 6/3. Should I anticipate around a 43 day cycle again as a basis for when to consider a “missed period” of it worked this cycle, or wait until 14 days after my estimated ovulation?