How to take an ovulation test?

Hi ladies, I'm new to taking ovulation tests so I just have a couple questions. ..

1. It says the urine and stick need to be at room temp. ...does that mean collect and let sit for a while?

2. If I have a 43 day avg cycle and am on day 10. when do you think I should start doing them? I did 1 this morning. ..collected. ..let sit for about 1/2 hour then tested. 1 dark control line and 1 faint test line. .... the test says that means is negative...

Thanks for any help!!!