Glow Pregnancy Test Guide

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How do pregnancy tests work?

Human chorionic gonadotropin (hCG) is a hormone, produced by the developing placenta and secreted into the urine.

The pregnancy test contains antibodies which specifically react with this hormone. When the sample end of the test is dipped in the urine specimen, the urine specimen is applied to the strip and migrates along the strip. When hCG in the sample reaches the Test Zone region of the membrane, it will form a colored line.

Absence of this colored line suggests a negative result. A colored line will appear at the Control Zone region if the test has been performed properly.

When should I take a pregnancy test?

hCG can often be detected by this test as early as the day after a missed period. Although any urine sample can be used for pregnancy testing, using the first morning urine is recommended for the most accurate results. This is because it contains the highest concentration of hCG.

How to use pregnancy tests:

How to read the results:

  • Positive (pregnant): Two distinct color lines appear—one in the Control Zone (C) and one in the Test Zone (T). This result indicates pregnancy.
  • Negative (not pregnant): Only one line appears in the Control Zone (C). This result indicates no pregnancy has been detected.
  • Invalid: No lines appear, or only the line in the Test Zone (T) appears. Repeat the test with a new test strip.

Need more information? Click here for our comprehensive instruction manual.